Analysis Note
ControlWestern Blot: GFP recombinant protein
Application
Research Sub CategoryEpitope Tags
Research CategorySecondary & Control Antibodies
Detect GFP using this Anti-GFP Antibody, clone 3F8.2 validated for use in WB.
Western Blot Analysis:
0.125 µg/mL - 1 µg/mL of this antibody detected GFP in 10 ng of GFP recombinant protein (Cat no. 14-392).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.
Immunogen
Epitope: Full Length
Full length GFP protein.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Protein G Purified
Purified mouse immunoglobulin in buffer containing PBS containing 0.05% sodium azide.
Format: Purified
Quality
Routinely evaluated by Western Blot on GFP recombinant protein.
Specificity
Reacts with most common vertebrate proteins containing the GFP tag.
The antibody reacts with the 28 kDa GFP protein.
Storage and Stability
Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Target description
28 kDa
This product has met the following criteria to qualify for the following awards: