ANTI-GFP

Code: MAB1083 D2-231

Analysis Note

ControlWestern Blot: GFP recombinant protein

Application

Research Sub CategoryEpitope Tags

Research CategorySecondary & Contr...


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Your Price
€386.00 EACH
€474.78 inc. VAT

Analysis Note

ControlWestern Blot: GFP recombinant protein

Application

Research Sub CategoryEpitope Tags

Research CategorySecondary & Control Antibodies

Detect GFP using this Anti-GFP Antibody, clone 3F8.2 validated for use in WB.

Western Blot Analysis:
0.125 µg/mL - 1 µg/mL of this antibody detected GFP in 10 ng of GFP recombinant protein (Cat no. 14-392).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.

Immunogen

Epitope: Full Length

Full length GFP protein.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Purified mouse immunoglobulin in buffer containing PBS containing 0.05% sodium azide.

Format: Purified

Quality

Routinely evaluated by Western Blot on GFP recombinant protein.

Specificity

Reacts with most common vertebrate proteins containing the GFP tag.

The antibody reacts with the 28 kDa GFP protein.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

28 kDa

antibody formpurified immunoglobulin
antibody product typeprimary antibodies
biological sourcemouse
clone3F8.2, monoclonal
isotypeIgG1κ
Quality Level100
shipped inwet ice
species reactivityvertebrates
technique(s)western blot: suitable
UniProt accession no.P42212
This product has met the following criteria to qualify for the following awards:



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